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描述
研究领域
Epigenetics, Infectious Diseases, Neuroscience
图片与验证
−| 已测应用 | FC, ICC, IHC-P, WB |
|---|---|
| 稀释范围 | Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. Flow cytometry: Recommended dilution: 1-5 µg/ml. Intracellular staining. Western blotting: Recommended dilution: 1 µg/ml. |
| 反应性 | Mammal |
| 应用说明 |
关键属性
−| 抗体类型 | Primary Antibody |
|---|---|
| 克隆性 | Monoclonal |
| 同种型 | Mouse IgM |
| 克隆号 | VI-01 |
| 免疫原 | Pellet of porcine brain cold stable proteins after depolymerization of microtubules. |
| 靶点 | Vimentin |
| 纯度 | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| 纯化 | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| 偶联 | Unconjugated |
储存与操作
−| 储存 | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| 缓冲液/防腐剂 | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| 浓度 | 1 mg/ml |
| 有效期 | 12 months from date of receipt. |
| 免责声明 | For research use only |
别名
−anti CTRCT30 antibody, anti Epididymis luminal protein 113 antibody, anti HEL113 antibody, anti VIM antibody, anti Vimentin antibody
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