EMI1 Antibody (Early Mitotic Inhibitor-1)

SKU: orb385841

描述

Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

研究领域

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics

图片与验证

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已测应用	IHC-P, WB
稀释范围	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
反应性	Human
应用说明	Optimal dilution of the EMI-1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2a, kappa
克隆号	FBXP5
免疫原	A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.
纯度	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Store the EMI-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
有效期	12 months from date of receipt.
危害信息	This EMI-1 antibody is available for research use only.
免责声明	For research use only

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应用：
FC, IF, IHC, WB
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EMI1 Antibody (Early Mitotic Inhibitor-1) [orb2641716]
应用：
IHC-P, WB
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Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

Q9UKT4

UniProt 详情

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暂无 UniProt 数据

实验方案信息

免疫印迹（WB）

查看实验方案

IHC-P

石蜡切片免疫组化