Human Heat Shock 70kDa Protein 1A (HSPA1A) ELISA Kit

SKU: orb775619

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1A(HSPA1A).

研究领域

Cancer Biology, Infectious Disease & Virology, Signal Transduction, Stem Cell & Developmental Biology

图片与验证

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Item 1 of 1

已测应用

ELISA

应用说明

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSPA1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSPA1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSPA1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSPA1A in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性	Human
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	1.57-100 ng/mL
灵敏度	0.6 ng/mL
浓度	100 ng/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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HSP70-1 ELISA Kit, HSP-A1A ELISA Kit, HSPA1 ELISA Kit, HSP70-2 ELISA Kit, HSX70 ELISA Kit

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质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

P0DMV8

UniProt 详情

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暂无 UniProt 数据

实验方案信息

ELISA

酶联免疫吸附测定（ELISA）

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Małgorzata Kowalska # 1, Marzena Tylicka # 2, Olga Martyna Koper-Lenkiewicz 3, Joanna Kamińska 3, Justyna Dorf 3, Ewa Matuszczak Plasma concentration of MMP-17 is elevated in boys with cryptorchidism and correlates with HSP-70 Sci Rep, (2024)

PubMed: 39747260

反应性：	Human
动态范围：	1.56-100ng/mL
灵敏度：	0.54ng/mL

反应性：	Human
动态范围：	125.00 - 8,000 pg/mL
灵敏度：	60 pg/mL