Human HeatShock Protein 70 (HSP-70/HSPA9) ELISA Kit

SKU: orb778378

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 70(HSP70).

研究领域

Cancer Biology, Infectious Disease & Virology, Stem Cell & Developmental Biology

图片与验证

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Item 1 of 1

已测应用

ELISA

应用说明

standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性	Human
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	3.13-200 ng/mL
灵敏度	1.39 ng/mL
浓度	200 ng/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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DnaK ELISA Kit, 70 Kilodalton Heat Shock Proteins ELISA Kit

质量保证

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常用数据库链接

UniProt

P38646

UniProt 详情

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暂无 UniProt 数据

实验方案信息