Human Interleukin 1 Beta (IL1b) ELISA Kit

SKU: orb775135

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta(IL1b).

研究领域

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

图片与验证

−

Item 1 of 1

已测应用

ELISA

应用说明

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1b in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

−

反应性	Human
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	15.63-1000 pg/mL
灵敏度	5.8 pg/mL
浓度	1000 pg/mL

储存与操作

−

储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

−

IL1-B ELISA Kit, IL1-Beta ELISA Kit, IL1F2 ELISA Kit, IL-1β ELISA Kit, Interleukin-1 Family Member 2 ELISA Kit, Catabolin ELISA Kit

相似产品

−

Item 1 of 1
Human Interleukin 1 Beta (IL-1β) High Sensitivity ELISA Kit [orb1945920]
反应性：
Human
动态范围：
0.16-10pg/mL
灵敏度：
0.09 pg/mL
可选规格： 96 T, 48 T
Item 1 of 1
Human pro interleukin 1 beta (pro-IL1β) ELISA Kit [orb1947846]
反应性：
Human
动态范围：
31.25-2000pg/mL
灵敏度：
18.75 pg/mL
可选规格： 48 T, 96 T
Item 1 of 1
Human Interleukin 1 Beta (IL-1β) Microsample Fast ELISA Kit [orb1806721]
反应性：
Human
动态范围：
7.82-500pg/mL
灵敏度：
4.69 pg/mL
可选规格： 48 T, 96 T
Item 1 of 1
Human Interleukin 1 Beta (IL-1β) ELISA Kit [orb1807418]
反应性：
Human
动态范围：
0.31-20pg/mL
灵敏度：
0.18 pg/mL
可选规格： 48 T, 96 T
Item 1 of 1
Human IL1 beta ELISA Kit [orb219432]
反应性：
Human
动态范围：
31.2 pg/ml - 2000 pg/ml
灵敏度：
10 pg/ml
可选规格： 96 T

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.