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Human Receptor Activator of Nuclear Factor-kB Ligand (RANKL) ELISA Kit

SKU: orb1496441
ELISA 试剂盒促销

描述

Human RANKL ELISA Kit

研究领域

Cancer Biology, Cell Biology, Metabolism Research, Musculoskeletal & Connective Tissue Research, Signal Transduction

图片与验证

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应用说明
standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RANKL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RANKL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RANKL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RANKL in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性Human
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
检测类型Sandwich
检测时间3.5h
范围78.13-5000 pg/mL
灵敏度23.6 pg/mL
浓度5000 pg/mL

储存与操作

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储存请参阅操作手册中的储存说明
有效期Please enquire.
免责声明For research use only

别名

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CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine; RANkL; Receptor Activator Of Nuclear Factor Kappa B Ligand

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UniProt 详情

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实验方案信息

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48 T
¥ 3,900.00
96 T
¥ 5,460.00
发货Usually dispatched within 5-10 working days
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