Mouse anti Human CD4, conjugated with Biotin

SKU: orb1570894

该产品可以定制

描述

研究领域

,Flow Cytometry

图片与验证

−

已测应用	FC
反应性	Human
应用说明	PBMC: Add10 µl of MAB/106 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN : (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use 5 µl of solution, rather than 10 µl as above. Excites at 488nm and emits at 670nm. Store protected from light.

关键属性

−

抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	IgG1
克隆号	7E14
免疫原	Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
偶联	Biotin

储存与操作

−

储存	Product should be stored at 4-8°C. DO NOT FREEZE
缓冲液/防腐剂	Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
浓度	Titered for flow cytometry
有效期	12 months from date of receipt.
危害信息	This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
免责声明	For research use only

别名

−

T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

P01730

UniProt 详情

−

暂无 UniProt 数据

实验方案信息

流式细胞术

查看实验方案

查看更多实验方案

Mouse anti Human CD4, conjugated with Biotin

描述

研究领域

图片与验证

关键属性

储存与操作

别名

质量保证

常用数据库链接

UniProt 详情

实验方案信息

可选规格