Mouse Claudin 5 (CLDN5) ELISA Kit

SKU: orb780989

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 5(CLDN5).

研究领域

Signal Transduction

图片与验证

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Item 1 of 1

已测应用

ELISA

应用说明

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CLDN5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CLDN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CLDN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CLDN5 in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性	Mouse
样本类型	Tissue homogenates, cell lysates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	0.32-20 ng/mL
灵敏度	0.119 ng/mL
浓度	20 ng/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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AWAL ELISA Kit, BEC1 ELISA Kit, CPETRL1 ELISA Kit, TMVCF ELISA Kit, Transmembrane Protein Deleted In Velocardiofacial Syndrome ELISA Kit, Transmembrane protein deleted in VCFS ELISA Kit

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Item 1 of 1
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动态范围：
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Mouse Claudin 5 ELISA Kit [orb407899]
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Mouse Claudin 5 (CLDN5) ELISA Kit [orb1667162]
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Mouse CLDN5 ELISA Kit [orb1197945]
反应性：
Mouse
动态范围：
0.312-20ng/mL
灵敏度：
0.127ng/mL
可选规格： 48 T, 96 T

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

O54942

UniProt 详情

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暂无 UniProt 数据

实验方案信息

ELISA

酶联免疫吸附测定（ELISA）

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反应性：	Mouse
动态范围：	31.25-2000pg/ml
灵敏度：	18.75pg/ml

反应性：	Mouse
动态范围：	18.75 pg/mL-1200 pg/mL
灵敏度：	4.68 pg/mL