Mouse Interleukin 18 (IL18) ELISA Kit

SKU: orb776133

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18(IL18).

研究领域

Immunology & Inflammation, Infectious Disease & Virology

图片与验证

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Item 1 of 1

已测应用

ELISA

应用说明

standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL18 in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性	Mouse
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	125-8000 pg/mL
灵敏度	37.9 pg/mL
浓度	8000 pg/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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IGIF ELISA Kit, IL-1g ELISA Kit, IL1F4 ELISA Kit, IL1-F4 ELISA Kit, Interferon-Gamma-Inducing Factor ELISA Kit, Interleukin-1 Family Member 4 ELISA Kit, Iboctadekin ELISA Kit, Interleukin-1 Gamma ELISA Kit

相似产品

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Mouse IL-18 ELISA Kit [orb565959]
反应性：
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Mouse IL18 ELISA Kit [orb408925]
反应性：
Mouse
动态范围：
1.56 pg/mL-100 pg/mL
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Mouse IL18 ELISA Kit [orb437211]
反应性：
Mouse
动态范围：
15.625-1000pg/mL
灵敏度：
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可选规格： 48 T, 96 T

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

P70380

UniProt 详情

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暂无 UniProt 数据

实验方案信息