Mouse NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) ELISA Kit

SKU: orb782216

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLR Family, Pyrin Domain Containing Protein 3(NLRP3).

研究领域

Signal Transduction

图片与验证

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Item 1 of 1

已测应用

ELISA

应用说明

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NLRP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NLRP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NLRP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NLRP3 in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性	Mouse
样本类型	Tissue homogenates, cell lysates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	0.16-10 ng/mL
灵敏度	0.052 ng/mL
浓度	10 ng/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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AVP ELISA Kit, AGTAVPRL ELISA Kit, AII ELISA Kit, AII/AVP ELISA Kit, C1orf7 ELISA Kit, CIAS1 ELISA Kit, CLR1.1 ELISA Kit, FCAS ELISA Kit, FCU ELISA Kit, MWS ELISA Kit, NALP3 ELISA Kit, PYPAF1 ELISA Kit, Cryopyrin ELISA Kit, Cold Induced Autoinflammatory Syndrome 1 ELISA Kit, Caterpiller-Like Receptor 1.1 ELISA Kit

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