Mouse PPARgC1a(Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha) Microsample ELISA Kit

SKU: orb2997781

描述

图片与验证

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应用说明

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PPARgC1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPARgC1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PPARgC1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PPARgC1a in the samples is then determined by comparing the OD of the samples to the standard curve.

关键属性

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样本类型	Tissue homogenates, cell lysates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	0.16-10 ng/mL
灵敏度	0.056 ng/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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LEM6; PGC-1(alpha); PGC-1v; PGC1; PGC1A; PPARGC1; Ligand effect modulator 6

质量保证

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常用数据库链接

UniProt

O70343

UniProt 详情

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暂无 UniProt 数据

实验方案信息

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Mouse PPARgC1a(Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha) Microsample ELISA Kit

描述

图片与验证

关键属性

储存与操作

别名

质量保证

常用数据库链接

UniProt 详情

实验方案信息

可选规格