Nucleophosmin Antibody / NPM1

SKU: orb2642808

描述

Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.

图片与验证

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已测应用	ELISA, FACS, IF, IHC-P, WB
稀释范围	ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/ml
反应性	Human
应用说明	Optimal dilution of the Nucleophosmin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2b, kappa
克隆号	NPM1/3287
免疫原	A portion of amino acids 185-287 from the human protein was used as the immunogen for the Nucleophosmin antibody.
纯度	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
有效期	12 months from date of receipt.
危害信息	This Nucleophosmin antibody is available for research use only.
免责声明	For research use only

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ELISA, IF, IHC-P, WB
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ELISA, FACS, IF, IHC-P, WB
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ELISA, FACS, IF, IHC-P, WB
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Nucleophosmin Antibody / NPM1 [orb2642814]
应用：
ELISA, FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg

质量保证

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