Pig Follicle Stimulating Hormone Receptor (FSHR) ELISA Kit

SKU: orb782256

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follicle Stimulating Hormone Receptor(FSHR).

研究领域

Stem Cell & Developmental Biology

图片与验证

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Item 1 of 1

已测应用

ELISA

应用说明

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig FSHR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig FSHR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig FSHR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig FSHR in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性	Porcine
样本类型	tissue homogenates, cell lysates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	0.32-20 ng/mL
灵敏度	0.125 ng/mL
浓度	20 ng/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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FSHRO ELISA Kit, LGR1 ELISA Kit, ODG1 ELISA Kit, FSH-Receptor ELISA Kit, Follitropin receptor ELISA Kit

质量保证

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常用数据库链接

UniProt

P49059

UniProt 详情

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暂无 UniProt 数据

实验方案信息