Rat Heat Shock Protein Beta 8 (HSPb8) ELISA Kit

SKU: orb780041

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 8(HSPb8).

研究领域

Neuroscience, Signal Transduction, Stem Cell & Developmental Biology

图片与验证

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Item 1 of 1

已测应用

ELISA

应用说明

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSPb8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSPb8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSPb8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSPb8 in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性	Rat
样本类型	Tissue homogenates, cell lysates and other biological fluids.
检测类型	Sandwich
检测时间	3.5h
范围	1.57-100 ng/mL
灵敏度	0.59 ng/mL
浓度	100 ng/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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CMT2L ELISA Kit, DHMN2 ELISA Kit, E2IG1 ELISA Kit, H11 ELISA Kit, HMN2 ELISA Kit, HSP22 ELISA Kit, Heat Shock 22kDa Protein 8 ELISA Kit, Alpha-crystallin C chain ELISA Kit, E2-induced gene 1 protein ELISA Kit, Small stress protein-like protein HSP22 ELISA Kit

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反应性：
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质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

Q9EPX0

UniProt 详情

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暂无 UniProt 数据

实验方案信息