Rat NitricOxide Synthase 2, Inducible (NOS2/iNOS) ELISA Kit

SKU: orb775367

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 2, Inducible(NOS2).

研究领域

Cancer Biology, Cardiovascular Research, Metabolism Research, Protein Biochemistry

图片与验证

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Item 1 of 1

已测应用

ELISA

应用说明

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性	Rat
样本类型	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	1.57-100 ng/mL
灵敏度	0.62 ng/mL
浓度	100 ng/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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NOS2A ELISA Kit, INOS ELISA Kit, HEP-NOS ELISA Kit, I-NOS ELISA Kit, Hepatocytes Oxide Synthase ELISA Kit, Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

Q06518

UniProt 详情

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暂无 UniProt 数据

实验方案信息