Rat Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide (PIK3Ca) ELISA Kit

SKU: orb2667103

描述

研究领域

Protein Biochemistry

图片与验证

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应用说明

standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIK3Ca. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIK3Ca. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIK3Ca, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIK3Ca in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	31.25-2000 pg/mL
灵敏度	11.3 pg/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; CCM4; CLAPO; CLOVE; CWS5; MCAP; MCM; MCMTC; PI3K; PI3K-alpha; p110-alpha

质量保证

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实验方案信息

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Rat Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide (PIK3Ca) ELISA Kit

描述

研究领域

图片与验证

关键属性

储存与操作

别名

质量保证

实验方案信息

可选规格