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Rat Phosphotylinosital 3 kinase (PI3K) ELISA Kit

SKU: orb782226
ELISA 试剂盒促销

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PI3K(Phosphotylinosital 3 kinase).

研究领域

Protein Biochemistry

图片与验证

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已测应用ELISA
应用说明
standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PI3K. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PI3K. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PI3K, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PI3K in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性Rat
样本类型Tissue homogenates and other biological fluids.
检测类型Sandwich
检测时间3.5h
范围15.63-1000 pg/mL
灵敏度3.9 pg/mL
浓度1000 pg/mL

储存与操作

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储存请参阅操作手册中的储存说明
有效期Please enquire.
免责声明For research use only

别名

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PI3K ELISA Kit, Phosphotylinosital 3 kinase ELISA Kit, ELISA Kit

相似产品

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

实验方案信息

ELISA
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Raghda N El Mahdy 1, Manar A Nader 1, Manar G Helal 1, Sally E Abu-Risha 2, Marwa E Abdelmageed Tiron ameliorates acetic acid-induced colitis in rats: Role of TGF-β/EGFR/PI3K/NF-κB signaling pathway Int Immunopharmacol ., (2024)

应用

ELISA

反应性

Rat

可选规格

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48 T
¥ 3,900.00
96 T
¥ 5,460.00
发货Usually dispatched within 5-10 working days
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