Vinculin Antibody

SKU: orb2644421

描述

This antibody recognizes both Vinculin (125kDa) and meta-Vinculin (135kDa). Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

图片与验证

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已测应用	FACS, IF, IHC-P
稀释范围	Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
反应性	Human
应用说明	Optimal dilution of the Vinculin antibody should be determined by the researcher.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG1, kappa
克隆号	VCL/3617
免疫原	Recombinant full-length human Vinculin protein was used as the immunogen for the Vinculin antibody.
纯度	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
有效期	12 months from date of receipt.
危害信息	This Vinculin antibody is available for research use only.
免责声明	For research use only

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FACS, IF, IHC-P
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应用：
ELISA, IF, IHC-P, WB
预测反应性：
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反应性：
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动态范围：
78.13-5000 pg/mL
灵敏度：
30 pg/mL
可选规格： 48 T, 96 T

质量保证

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