Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit

SKU: orb1807472

特色

描述

研究领域

Metabolism Research, Musculoskeletal & Connective Tissue Research, Stem Cell & Developmental Biology

图片与验证

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Item 1 of 1

应用说明

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

关键属性

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反应性	Human
样本类型	serum, plasma, Tissue homogenate and Other biological samples
检测类型	Sandwich
检测时间	3.5H
范围	31.25-2000pg/mL
灵敏度	13.7 pg/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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HMGB1, HMG1, HMG3, SBP-1, Amphoterin

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Item 1 of 1
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反应性：
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动态范围：
62.5-4000 pg/mL
灵敏度：
26.9 pg/mL
可选规格： 48 T, 96 T
Human HMGB1 ELISA Kit [orb406327]
反应性：
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动态范围：
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Human HMGB-1 ELISA Kit [orb1149568]
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动态范围：
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Human High Mobility Group Protein B1 ELISA Kit [orb1214273]
反应性：
Human
动态范围：
0.5-150ng/ml
灵敏度：
0.24ng/ml
可选规格： 48 T, 96 T

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

基因符号

HMGB-1

UniProt

P09429

UniProt 详情

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暂无 UniProt 数据

实验方案信息

查看更多实验方案

按应用筛选

ELISA (1)

按种属筛选

Human (1)

Yun He 1 2, Di Zhang 1, Jiao Liu 1, Junwen Ouyang 1, Chengxiao Zhang 1, Qian Tan 2, Jiaxuan Jiang 1, Kai Hu Ocular Soluble Microneedle Patch Loaded with Immunogenic Cell Death Inducer PKHB1 Peptide for Alleviating Herpes Simplex Keratitis ACS Appl Mater Interfaces, (2025)

PubMed: 40685578

应用

ELISA

反应性

Human

反应性：	Human
动态范围：	62.5-4000 pg/mL
灵敏度：	26.9 pg/mL

Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit

描述

研究领域

图片与验证

关键属性

储存与操作

别名

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