Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit

SKU: orb775302

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1(HMG1).

研究领域

Infectious Disease & Virology

图片与验证

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Item 1 of 1

已测应用

ELISA

应用说明

standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性	Human
样本类型	serum, plasma and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	62.5-4000 pg/mL
灵敏度	26.9 pg/mL
浓度	4000 pg/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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HMGB1 ELISA Kit, HMG3 ELISA Kit, SBP1 ELISA Kit, Sulfoglucuronyl Carbohydrate Binding Protein ELISA Kit, Amphoterin ELISA Kit, High Mobility Group Box 1 Protein ELISA Kit

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Item 1 of 1
Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit [orb1807472]
反应性：
Human
动态范围：
31.25-2000pg/mL
灵敏度：
13.7 pg/mL
可选规格： 96 T, 48 T
Human HMGB1 ELISA Kit [orb406327]
反应性：
Human
动态范围：
78 pg/mL-5000 pg/mL
灵敏度：
19.5 pg/mL
可选规格： 10 x 96 T, 5 x 96 T, 48 T, 96 T, 24 T
Human HMGB-1 ELISA Kit [orb1149568]
反应性：
Human
动态范围：
1250pg/ml-40000pg/ml
灵敏度：
10pg/ml
可选规格： 96 T, 48 T
Human High Mobility Group Protein B1 ELISA Kit [orb1214273]
反应性：
Human
动态范围：
0.5-150ng/ml
灵敏度：
0.24ng/ml
可选规格： 48 T, 96 T

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

P09429

UniProt 详情

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暂无 UniProt 数据

实验方案信息

ELISA

酶联免疫吸附测定（ELISA）

查看实验方案

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按应用筛选

ELISA (1)

按种属筛选

Human (1)

Mahdieh Azizi 1, Alireza Andalib 2, Marzieh Rezaei Investigating the potential role of miR-451a as a tumor suppressor in immunogenic cell death induction and dendritic cell maturation in colorectal cancer cell lines Cancer Cell Int ., (2025)

PubMed: 40770807

应用

ELISA

反应性

Human

反应性：	Human
动态范围：	31.25-2000pg/mL
灵敏度：	13.7 pg/mL

Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit

描述

研究领域

图片与验证

关键属性

储存与操作

别名

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