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Mouse anti Human CD4

SKU: orb1570895
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描述

Mouse anti Human CD4

研究领域

,Flow Cytometry

图片与验证

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Mouse anti Human CD4
已测应用FC
反应性Human
应用说明
PBMC: Add10 µl of MAB/10 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light.

关键属性

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抗体类型Primary Antibody
宿主Mouse
克隆性Monoclonal
同种型IgG1
克隆号7E14
免疫原Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
偶联Unconjugated

储存与操作

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储存Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂Provided as solution in phosphate buffered saline with 0.08% sodium azide
浓度See vial for concentration
有效期12 months from date of receipt.
危害信息This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
免责声明For research use only

别名

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T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

相似产品

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt
P01730

UniProt 详情

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暂无 UniProt 数据

实验方案信息

FC
流式细胞术
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可选规格

请在下方选择规格

100 μg
¥ 4,680.00
选择偶联或无载体版本
免费二抗（20 µl）0/0

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发货Usually dispatched within 5-10 working days
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