Mouse Cluster of Differentiation 40 Ligand (CD40L) ELISA Kit

SKU: orb1947207

特色

描述

研究领域

Cardiovascular Research

图片与验证

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Item 1 of 1

应用说明

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD40L. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD40L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD40L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CD40L. You can calculate the concentration of Mouse CD40L in the samples by comparing the OD of the samples to the standard curve.

关键属性

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反应性	Mouse
样本类型	serum, plasma, Tissue homogenate and Other biological samples
检测类型	Sandwich
检测时间	3.5H
范围	93.75-6000pg/mL
灵敏度	56.25 pg/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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TNFSF5, CD4LG, CD154, HIGM1, IGM, IMD3, T-BAM, TRAP, Gp39

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反应性：
Mouse
动态范围：
15.625-1000pg/mL
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质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

基因符号

CD40L

UniProt

P27548

UniProt 详情

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暂无 UniProt 数据

实验方案信息

查看更多实验方案

反应性：	Mouse
动态范围：	15.63-1000 pg/mL
灵敏度：	6.8 pg/mL

反应性：	Mouse
动态范围：	0.274 ng/ml-200 ng/ml.
灵敏度：	0.274 ng/ml.

Mouse Cluster of Differentiation 40 Ligand (CD40L) ELISA Kit

描述

研究领域

图片与验证

关键属性

储存与操作

别名

相似产品