Mouse Cluster of Differentiation 40 Ligand (CD40L) ELISA Kit

SKU: orb776172

特色

描述

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 40 Ligand(CD40L).

研究领域

Cancer Biology, Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction

图片与验证

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Item 1 of 1

已测应用

ELISA

应用说明

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD40L in the samples is then determined by comparing the OD of the samples to the standard curve

关键属性

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反应性	Mouse
样本类型	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
检测类型	Sandwich
检测时间	3.5h
范围	15.63-1000 pg/mL
灵敏度	6.8 pg/mL
浓度	1000 pg/mL

储存与操作

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储存	请参阅操作手册中的储存说明
有效期	Please enquire.
免责声明	For research use only

别名

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CD154 ELISA Kit, TRAP ELISA Kit, HIGM1 ELISA Kit, IGM ELISA Kit, IMD3 ELISA Kit, TBAM ELISA Kit, T-BAM ELISA Kit, TNFSF5 ELISA Kit, Gp39 ELISA Kit, TNF Superfamily Member 5 ELISA Kit, Hyper-IgM Syndrome ELISA Kit, TNF-Related Activation Protein ELISA Kit, T-Cell B-Cell Activating Molecule ELISA Kit

相似产品

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Mouse Cluster of Differentiation 40 Ligand (CD40L) ELISA Kit [orb1947207]
反应性：
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动态范围：
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Mouse CD40LG ELISA Kit [orb409206]
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Mouse Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit [orb1667327]
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Mouse CD40L ELISA Kit (Ready to Use) [orb553171]
反应性：
Mouse
动态范围：
15.6-1000pg/mL
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Mouse CD40L ELISA Kit [orb438502]
反应性：
Mouse
动态范围：
15.625-1000pg/mL
灵敏度：
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可选规格： 96 T, 48 T

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

P27548

UniProt 详情

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暂无 UniProt 数据

实验方案信息