CD44 Antibody / HCAM

SKU: orb2637605

描述

This antibody recognizes a cell surface glycoprotein of 80-95kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44-s). Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

图片与验证

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已测应用	FACS, IF, IHC-P, WB
稀释范围	Flow cytometry: 2-4ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
反应性	Human
应用说明	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCAM antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2a, kappa
克隆号	156-3C11
免疫原	Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
纯度	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
有效期	12 months from date of receipt.
危害信息	This HCAM antibody is available for research use only.
免责声明	For research use only

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Item 1 of 5
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应用：
FACS, IF, IHC-P, WB
反应性：
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克隆性：
Monoclonal
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CD44 Antibody / HCAM [orb749903]
应用：
FACS, IF, IHC-P, WB
反应性：
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可选规格： 20 μg
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CD44 Antibody / HCAM [orb749904]
应用：
IF, IHC-P, WB
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Human, Mouse
种属/宿主：
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克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg
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应用：
FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
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Recombinant
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Unconjugated
可选规格： 20 μg
Item 1 of 4
CD44 Antibody / HCAM [orb2639487]
应用：
FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.