CD44 Antibody / HCAM

SKU: orb749904

描述

Recognizes a cell surface glycoprotein of 80-95kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

研究领域

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics

图片与验证

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Item 1 of 4

已测应用	IF, IHC-P, WB
稀释范围	Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
反应性	Human, Mouse
应用说明	Optimal dilution of the CD44 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG1, kappa
克隆号	DF1485
免疫原	Purified CD44 antigen (PGp-1) from lymphocyte membrane was used as the immunogen for the CD44 antibody.
纯度	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Store the CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
有效期	12 months from date of receipt.
危害信息	This CD44 antibody is available for research use only.
免责声明	For research use only

相似产品

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Item 1 of 5
CD44 Antibody / HCAM [orb749333]
应用：
FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg
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CD44 Antibody / HCAM [orb2637605]
应用：
FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg
Item 1 of 4
CD44 Antibody / HCAM [orb749903]
应用：
FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg
Item 1 of 4
CD44 Antibody / HCAM [orb640057]
应用：
FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Recombinant
偶联：
Unconjugated
可选规格： 20 μg
Item 1 of 4
CD44 Antibody / HCAM [orb2639487]
应用：
FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

P16070

UniProt 详情

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暂无 UniProt 数据

实验方案信息

免疫印迹（WB）

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IHC-P

石蜡切片免疫组化

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免疫荧光

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应用：	FACS, IF, IHC-P, WB
反应性：	Human
种属/宿主：	Mouse
克隆性：	Monoclonal
偶联：	Unconjugated

CD44 Antibody / HCAM

描述

研究领域

图片与验证

关键属性

储存与操作

相似产品