NSE Antibody / Neuron Specific Enolase

SKU: orb386134

描述

Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

研究领域

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics

图片与验证

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已测应用	IHC-P, WB
稀释范围	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.2-0.4ug/ml for 30 min at RT
反应性	Human, Mouse, Rat
应用说明	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2b, kappa
克隆号	ENO2/1375
免疫原	Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.
纯度	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
有效期	12 months from date of receipt.
危害信息	This NSE antibody is available for research use only.
免责声明	For research use only

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IHC-P, WB
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应用：
IHC-P, WB
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NSE Antibody / Neuron Specific Enolase [orb1825911]
应用：
IHC-P, WB
反应性：
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种属/宿主：
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偶联：
Unconjugated
可选规格： 20 μg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

P09104

NCBI 基因 ID

2026

UniProt 详情

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暂无 UniProt 数据

NCBI 基因详情

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暂无 NCBI 基因数据

实验方案信息

免疫印迹（WB）

查看实验方案

IHC-P

石蜡切片免疫组化