NSE Antibody / Neuron Specific Enolase

SKU: orb750096

描述

This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

研究领域

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics

图片与验证

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已测应用	IHC-P
稀释范围	Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
反应性	Human, Mouse, Rat
应用说明	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Neuron Specific Enolase antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2b, kappa
克隆号	ENO2/1462
免疫原	Amino acids 416-433 of human NSE were used as the immunogen for this Neuron Specific Enolase antibody.
纯度	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Store the Neuron Specific Enolase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
有效期	12 months from date of receipt.
危害信息	This Neuron Specific Enolase antibody is available for research use only.
免责声明	For research use only

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NSE Antibody / Neuron Specific Enolase [orb1825911]
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IHC-P, WB
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质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

P09104

UniProt 详情

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暂无 UniProt 数据

实验方案信息

IHC-P

石蜡切片免疫组化

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